If it weren't for the pistol, I would've guessed somewhere like Australia. The huntsman spiders they have down there get huge. Not really poisonous, but they can still inflict a fairly painful bite.Doesn't say where this is, but if in the US, likely a wolf spider or nursery web spider, harmless.
And those eyes don't glow by themselves - they shine by reflected light.
But to some, NO spider is harmless.